Study Service Details:
Multiplex (Multicolor) IHC and IHC/ISH for simultaneous
detection of co-localized biomarkers in individual cells
Tissue Preparation and Sectioning:
If you have not collected your specimens yet, our scientists can provide guidance on best practices for collection, storage and fixation (if done at your end) of human or animal tissue. You can provide us with fresh or frozen tissue – we’ll perform quality control, tissue fixation for fresh tissue, processing, trimming, embedding and sectioning. We’ll select the right microtome for each application so that we have the best FFPE samples for your studies, and we’ll employ microscopic analysis to ensure that we have the right type of tissue in the optimal orientation on the slides for analysis. We can also create tissue microarrays (TMAs) if suited to your project. If you already have prepared sections or FFPE slides ready for analysis, just send us your samples.
Chromogenic Multiplexed Immunohistochemistry (ChroMIHC™):
Single color fluorescent or chromogenic stains work fine for studying markers one at a time in tissue. These methods cannot detect multiple cell types or differential co-localized marker expression without utilizing a large number of precious sequential tissue sections, and even then may miss critical microenvironment boundaries. Gnome Sciences’ ChroMIHCTM (a trademark of Gnome Sciences) enables simultaneous detection of multiple markers at the cellular/sub-cellular level within individual tissue sections. Our solution uses distinct differentially labeled antibodies for the multiplexed IHC to reveal marker colocalization and cell type identification. We can combine chromogenic stains as well.
Antibody selection and optimization:
Gnome Sciences’ scientists can help you select the right antibodies and labels for your study, and we can also develop and validate the detection protocols. We will work with you every step of the way, and we can take any or all of your project needs. We specialize in performing the complicated ‘difficult studies’ that the big players stay away from (but we are happy to help you with routine projects as well).
Gnome Sciences has expertise in multiple detection and analysis methodologies and can mix and match these techniques to get you the answers you need. For example, we can combine chromogenic and fluorogenic marker detection to enable simultaneous protein and RNA expression/colocalization. We can combine anatomical pathology with IHC, RNA microRNA and other molecular pathology detection techniques in single sections. We can provide gene variant analysis to compare patients or study cellular differences in tumor microenvironments. We offer viral RNA detection and variant analysis for COVID-19 biospecimens and other viral infectious diseases. We can combine protein marker analysis with quantitative gene expression analysis (qRT-PCR) and laser capture microscopy (LCM) for single cell analysis of genes and cells. Tell us what you want to accomplish, and we will work with you to get the answers.